Trifluoroacetic acid → HCID 71279946, compound

HRID 246781Notes

Reaction equation

HRID 246781 reaction equation
[F:1][C:2]1[CH:10]=[C:9]2[C:5]([C:6]([C:12]3[N:17]=[C:16]4[C:18]([C:21]([NH:23][CH2:24][C:25]5([OH:36])[CH2:28][N:27](C(OC(C)(C)C)=O)[CH2:26]5)=[O:22])=[CH:19][NH:20][C:15]4=[N:14][CH:13]=3)=[N:7][N:8]2[CH3:11])=[CH:4][CH:3]=1.[F:37][C:38]([F:43])([F:42])[C:39]([OH:41])=[O:40]>ClCCl>[F:37][C:38]([F:43])([F:42])[C:39]([OH:41])=[O:40].[F:1][C:2]1[CH:10]=[C:9]2[C:5]([C:6]([C:12]3[N:17]=[C:16]4[C:18]([C:21]([NH:23][CH2:24][C:25]5([OH:36])[CH2:28][NH:27][CH2:26]5)=[O:22])=[CH:19][NH:20][C:15]4=[N:14][CH:13]=3)=[N:7][N:8]2[CH3:11])=[CH:4][CH:3]=1

Participants

4

Reactant 2

HCID 6422Trifluoroacetic acidHCID 71280759HCID 71280759, compound

Product 1

HCID 71279946HCID 71279946, compoundYield 9%

Reagents, catalysts & solvents 1

HCID 6344DichloromethaneSolvent

Procedure

To a solution of tert-butyl 3-((2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxamido)methyl)-3-hydroxyazetidine-1-carboxylate (86 mg, 0.16 mmol) in 3 mL of dichloromethane was added 3 mL of trifluoroacetic acid. The reaction mixture was stirred overnight at room temperature. After solvent evaporation, the residue was purified by preparative-HPLC (Gemini 5u C18 150×21.2 mm; inject volume: 3 mL/inj, flow rate: 20 mL/min; wavelength: 214 nm and 254 nm; the gradient conditions are: 40% acetonitrile/60% water (0.1% trifluoroacetic acid V/V) initially, and then proceed to 50% acetonitrile/50% water (0.1% trifluoroacetic acid V/V) in a linear fashion after just 9 min) to afford 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-N-((3-hydroxyazetidin-3-yl)methyl)-5H-pyrrolo[3,2-b]pyrazine-7-carboxamide trifluoroacetate (6 mg, 9%) as yellow solid. LCMS: (M+H)+=396; 1H NMR (300 MHz, CD3OD): δ 9.05 (s, 1H), 8.48-8.42 (m, 1H), 8.23 (s, 1H), 7.20 (d, 1H, J=9.0 Hz), 6.99 (t, 1H, J=9.3 Hz), 4.10-3.89 (m, 6H).

Workup

  1. customAfter solvent evaporation
  2. customthe residue was purified by preparative-HPLC (Gemini 5u C18 150×21.2 mm
  3. wait40% acetonitrile/60% water (0.1% trifluoroacetic acid V/V) initially, and then proceed to 50% acetonitrile/50% water (0.1% trifluoroacetic acid V/V) in a linear fashion after just 9 min

Sources & evidence

ORD record
ord-92f061ce03a747328af4e3b2896e35c3
Source dataset
uspto-grants-2014_02
Patent number
US08658646B2

Reaction notes

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