反应详情

(4-{(2R,3R)-1-(4-Fluorophenyl)-4-oxo-3-[(2-oxo-2-phenylethyl)thio}azetidin-2-yl]phenoxy)acetic acid (15 mg, 0.043 mmol), tert-butyl glycyl-D-valinate hydrochloride, (14.3 mg, 0.054 mmol) and N-methylmorpholine (14 μl, 0.13 mmol) were dissolved in DCM (2 ml). After 5 minutes, TBTU (16.9 mg, 0.053 mmol) was added and the reaction mixture was stirred for 2.5 h. The formation of the ester was confirmed. M/z: 678.35 (N+H). The solvent was removed under reduced pressure. The residue was dissolved in EtOAc:DCM (1:3) and purified on silica gel (1 g) using EtOAc:DCM (1:3) as eluent. The fractions were collected and concentrated. The residue (0.029 g) was dissolved in DCM (3 ml) and TFA (0.5 ml) was added. The reaction mixture was stirred overnight. The solvent was removed under reduced pressure and toluene was added and removed under reduced pressure. The yellowish residue was dissolved in MeOH (2 ml) and sodium borohydride (16.2 mg, 0.43 mmol) was added. The reaction mixture was stirred for 10 minutes. Ammonium acetate (31.4 mg) was added and the solvent was removed under reduced pressure. The residue was purified with preparative HPLC on a C8 column. A gradient from 20% to 50% MeCN in 0.1 M ammonium acetate buffer was used as eluent. After lyophilisation, the title compound was obtained as a white solid (7.6 mg, 28%). H-NMR (400 MHz, DMSO-d6): 0.79 (d, 3H), 0.81 (d, 3H) 1.95-2.05 (m, 1H), 2.84-2.96 (m, 2H), 3.79 (d, 2H), 3.98-4.04 (m, 1H), 4.27 (d, 0.5H), 4.30 (d, 0.5H), 4.51 (s, 2H), 4.66-4.75 (m, 1H), 5.02 (d, 0.5H), 5.04 (d, 0.5H), 6.98 (d, 2H), 7.10-7.17 (m, 2H), 7.19-7.32 (m, 7H), 7.36 (d, 2H), 7.77 (t, 1H), 8.26 (t, 1H). M/z: 622.1 (M−H) and 624.2 (M+H).

1. customThe solvent was removed under reduced pressure
2. dissolutionThe residue was dissolved in EtOAc:DCM (1:3)
3. custompurified on silica gel (1 g)
4. customThe fractions were collected
5. concentrationconcentrated
6. dissolutionThe residue (0.029 g) was dissolved in DCM (3 ml)
7. additionTFA (0.5 ml) was added
8. stirringThe reaction mixture was stirred overnight
9. customThe solvent was removed under reduced pressure and toluene
10. additionwas added
11. customremoved under reduced pressure
12. dissolutionThe yellowish residue was dissolved in MeOH (2 ml)
13. stirringThe reaction mixture was stirred for 10 minutes
14. customthe solvent was removed under reduced pressure
15. customThe residue was purified with preparative HPLC on a C8 column