4-Methylmorpholine → HCID 66842603, compound

HRID 254059Notes

Reaction equation

HRID 254059 reaction equation
[F:1][C:2]1[CH:7]=[CH:6][C:5]([N:8]2[C:11](=[O:12])[C@H:10]([S:13][CH2:14][C:15]([C:17]3[CH:22]=[CH:21][C:20]([F:23])=[CH:19][CH:18]=3)=[O:16])[C@H:9]2[C:24]2[CH:38]=[CH:37][C:27]([O:28][CH2:29][C:30]([NH:32][CH2:33][C:34](O)=[O:35])=[O:31])=[CH:26][CH:25]=2)=[CH:4][CH:3]=1.CN1CCOCC1.CN(C(ON1N=NC2C=CC=CC1=2)=[N+](C)C)C.[B-](F)(F)(F)F.[C:68]([CH2:72][C@H:73]([C:75]([OH:77])=[O:76])[NH2:74])([CH3:71])([CH3:70])[CH3:69].[BH4-].[Na+]>CN(C=O)C.CO.O>[F:1][C:2]1[CH:3]=[CH:4][C:5]([N:8]2[C:11](=[O:12])[C@H:10]([S:13][CH2:14][CH:15]([C:17]3[CH:22]=[CH:21][C:20]([F:23])=[CH:19][CH:18]=3)[OH:16])[C@H:9]2[C:24]2[CH:25]=[CH:26][C:27]([O:28][CH2:29][C:30]([NH:32][CH2:33][C:34]([NH:74][C@@H:73]([C:75]([OH:77])=[O:76])[CH2:72][C:68]([CH3:71])([CH3:70])[CH3:69])=[O:35])=[O:31])=[CH:37][CH:38]=2)=[CH:6][CH:7]=1

Participants

9

Reactant 5

HCID 79724-MethylmorpholineHCID 2733207O-(Benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium tetrafluoroborateHCID 4311764Sodium borohydrideHCID 69505084-Methyl-D-leucineHCID 66842760N-{[4-((2R,3R)-1-(4-Fluorophenyl)-3-{[2-(4-fluorophenyl)-2-oxoethyl]thio)-4-oxoazetidin-2-yl)phenoxy]acetyl}glycine

Product 1

HCID 66842603HCID 66842603, compoundYield 70.6%

Reagents, catalysts & solvents 3

HCID 887MethanolSolventHCID 962WaterSolventHCID 6228DimethylformamideSolvent

Procedure

N-{[4-((2R,3R)-1-(4-Fluorophenyl)-3-{[2-(4-fluorophenyl)-2-oxoethyl]thio}-4-oxoazetidin-2-yl)phenoxy]acetyl}glycine (20 mg, 0.037 mmol) was dissolved in 1.5 ml DMF. N-Methylmorpholine (13 μl, 118 mmol) and TBTU (15 mg, 0.047 mmol) were added and the mixture was stirred for 1 h., β-tert-Butyl-D-alanine (11 mg, 0.076 mmol) was added and the mixture was stirred for 3 h. Water (0.2 ml) was added and the mixture was stirred for 15 min. MeOH (2 ml) and NaBH4 (15 mg) were added. NH4Ac (ca 20 mg) was added after 15 min. Purification was performed using preparative HPLC on a C8 column. A gradient from 20-50% MeCN in 0.1M NH4Ac was used as mobile phase. Lyophilization yielded 17.5 mg (70%) of the title compound. M/z: 668 (M−1). NMR (400 MHz, DMSO): 8.24 (t, 1H), 7.77-7.87 (m, 1H), 7.32-7.40 (m, 4H), 7.22-7.27 (m, 2H), 7.07-7.19 (m, 4H), 6.99 (d, 2H), 5.06 (dd, 1H), 4.70-4.78 (m, 1H), 4.53 (s, 2H), 4.31 (dd, 1H), 4.09-4.17 (m, 1H), 3.65-3.81 (m, 2H), 2.84-3.00 (m, 2H), 1.65 (dd, 1H), 1.42 (dd, 1H), 0.86 (s, 9H).

Workup

  1. stirringthe mixture was stirred for 3 h
  2. stirringthe mixture was stirred for 15 min
  3. additionNH4Ac (ca 20 mg) was added after 15 min
  4. customPurification

Sources & evidence

ORD record
ord-73fc40a8b7394838b283ee3229a7dbd1
Source dataset
uspto-grants-2011_01
Patent number
US07871998B2

Reaction notes

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