Fitc dmf → Fluorescein 5-isothiocyanate

HRID 255469Notes

Reaction equation

HRID 255469 reaction equation
[CH:1]1[C:6]([N:7]=[C:8]=[S:9])=[CH:5][C:4]2[C:10]([O:12][C:13]3([C:23]4[CH:24]=[CH:25][C:26]([OH:28])=[CH:27][C:22]=4[O:21][C:15]4[CH:16]=[C:17]([OH:20])[CH:18]=[CH:19][C:14]3=4)[C:3]=2[CH:2]=1)=[O:11].CN(C=O)C>CN(C=O)C>[CH:1]1[C:6]([N:7]=[C:8]=[S:9])=[CH:5][C:4]2[C:10]([O:12][C:13]3([C:14]4[CH:19]=[CH:18][C:17]([OH:20])=[CH:16][C:15]=4[O:21][C:22]4[CH:27]=[C:26]([OH:28])[CH:25]=[CH:24][C:23]3=4)[C:3]=2[CH:2]=1)=[O:11]

Participants

3

Reactant 1

HCID 131714840Fitc dmf

Product 1

HCID 18730Fluorescein 5-isothiocyanate

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent2×

Procedure

A solution of 5.8 mg of FITC in 4 ml of DMF was prepared and serially diluted 1:4 with DMF. To 0.3 ml of each FITC-DMF dilution was added 5 ml of the above 1% microbead suspension. The suspensions were stirred for 1 hour and the equivalent fluorescence of each was determined. The results are given in the following Table:

Sources & evidence

ORD record
ord-eb50c578b05340d6bdb6f32610e24de4
Source dataset
uspto-grants-1987_10
Patent number
US04698262

Reaction notes

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