反应详情

To a solution of 72 mg of the product of Step C dissolved in 2 mL of methanol, was added 0.25 mL of a 1N solution of NaOH and the reaction mixture was stirred at room temperature for 3 days. The reaction mixture was then adjusted to pH 7 with 1N HCl, caoncentrated in vacuo, and the residue was applied to a silica gel flash chromatography column and eluted with CHCl3 /MeOH/NH4OH (80:15:1). Evaporation of the purified fractions and drying in vacuo afforded 50 mg (71%) of the title compound.

1. washeluted with CHCl3 /MeOH/NH4OH (80:15:1)
2. customEvaporation of the purified fractions
3. customdrying in vacuo