HuagongsheHuagongshe Chemistry Print Report · For research reference only
Nadh → Cotinine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Nadh | HCID 439153 | — |
| Reactant | Beta-D-Glucose 6-phosphate | HCID 439427 | — |
| Reactant | Cotinine | HCID 854019 | — |
| Product | Cotinine | HCID 854019 | — |
| Product | glucose-6-phosphate NAD | HCID 131719436 | — |
Reaction Steps
Cotinine-enzyme conjugate was prepared at 1 mg/ml of protein and diluted 500-fold to a final concentration of 2.0 μg/ml. The anti-cotinine antibody was present in stock solution at an approximate concentration of 15 mg/ml. The glucose-6-phosphate-NAD solution was prepared by mixing 3 volumes of 0.11M glucose-6-phosphate with 2 volumes of 0.1M NAD.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05527686 |
| Source dataset | uspto-grants-1996_06 |
| ORD ID | ord-81ea2360901b4ab6ad533dc50ccdf0ca |
| Source note | uspto-grants-1996_06 |