Urea → HCID 178418600, compound

Participants

Reaction Steps

Each of the strain AUL-6 and its parent strain FERM P-3616 was cultured at 30° C. for 24 hours on the BYG agar medium and then inoculated into a thick test tube charged with 6 ml of a seed medium (a medium prepared by dissolving 20 g of glucose, 10 g of peptone, 10 g of yeast extract and 2.5 g of sodium chloride in 1 liter of water and adjusting its pH to 7.2) and cultured at 30° C. for 24 hours. Next, 2 ml of the seed culture broth was inoculated into a 300 ml capacity conical flask charged with 20 ml of a main culture medium (a medium prepared by dissolving 150 g of blackstrap molasses (as glucose), 5 g of corn steep liquor, 30 g of ammonium sulfate, 3 g of urea, 0.5 g of dipotassium hydrogenphosphate, 0.5 g of potassium dihydrogenphosphate and 0.25 g of magnesium sulfate dihydrate in 1 liter of water, adjusting its pH to 7.2 and then adding 30 g of calcium carbonate thereto) and cultured at 30° C. for 72 hours.

Provenance & Evidence