Urea → HCID 178418600, compound

HRID 268590Notes

Reaction equation

HRID 268590 reaction equation
O=C[C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])O)O)O)O.S([O-])([O-])(=O)=O.[NH4+:18].[NH4+:19].[NH2:20][C:21]([NH2:23])=O.P([O-])([O-])(O)=O.[K+].[K+].P([O-])(O)(O)=O.[K+].[OH2:37].O.S([O-])([O-])(=O)=O.[Mg+2].C(=O)([O-])[O-].[Ca+2]>O>[NH2:18][C@H:9]([C:11]([OH:12])=[O:37])[CH2:7][CH2:5][CH2:3][NH:20][C:21](=[NH:23])[NH2:19]

Participants

9

Reactant 7

HCID 1176UreaHCID 10112Calcium CarbonateHCID 24450Potassium Phosphate, DibasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×HCID 516951Monobasic potassium phosphateHCID 6097028Ammonium SulfateHCID 22066319Magnesium sulfate dihydrate

Product 1

HCID 178418600HCID 178418600, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Each of the strain AUL-6 and its parent strain FERM P-3616 was cultured at 30° C. for 24 hours on the BYG agar medium and then inoculated into a thick test tube charged with 6 ml of a seed medium (a medium prepared by dissolving 20 g of glucose, 10 g of peptone, 10 g of yeast extract and 2.5 g of sodium chloride in 1 liter of water and adjusting its pH to 7.2) and cultured at 30° C. for 24 hours. Next, 2 ml of the seed culture broth was inoculated into a 300 ml capacity conical flask charged with 20 ml of a main culture medium (a medium prepared by dissolving 150 g of blackstrap molasses (as glucose), 5 g of corn steep liquor, 30 g of ammonium sulfate, 3 g of urea, 0.5 g of dipotassium hydrogenphosphate, 0.5 g of potassium dihydrogenphosphate and 0.25 g of magnesium sulfate dihydrate in 1 liter of water, adjusting its pH to 7.2 and then adding 30 g of calcium carbonate thereto) and cultured at 30° C. for 72 hours.

Workup

  1. custominoculated into a thick test tube
  2. additioncharged with 6 ml of a seed medium (a medium
  3. customprepared
  4. extractionextract
  5. waitcultured at 30° C. for 24 hours
  6. additioncharged with 20 ml of a main culture medium (a medium
  7. customprepared
  8. wait) and cultured at 30° C. for 72 hours

Sources & evidence

ORD record
ord-3e138327a2084ff3afcd0963888da80f
Source dataset
uspto-grants-2008_11
Patent number
US07445915B2

Reaction notes

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