反应详情

To a solution of 0.082 g (0.14 mmol) of the product of step C in 1.0 mL of methanol and 1.0 mL methylene chloride was added 250 gL of a 5.0 N solution of sodium hydroxide and the reaction was stirred at room temperature for 16 hours. The reaction mixture was then adjusted to pH=5-6 with dropwise addition of 6 N hydrochloric acid, and then concentrated in vacuo. The residue was redissolved in methanol, filtered, and evaporated. The mixture was then purified on a silica gel flash chromatography column eluted with CHCl3 --MeOH-NH4OH (80:15:1). Evaporation of the purified fractions and drying in vacuo afforded 0.061 g (90%) of the title compound.

1. concentrationconcentrated in vacuo
2. dissolutionThe residue was redissolved in methanol
3. filtrationfiltered
4. customevaporated
5. customThe mixture was then purified on a silica gel flash chromatography column
6. washeluted with CHCl3
7. customEvaporation of the purified fractions
8. customdrying in vacuo