反应详情

Pyrophosphoric acid (15 g/dl) and 8 g/dl of inosine were dissolved in an acetate buffer (pH 4.0). To the solution was added E. coli JM109 strain having introduced therein the above-mentioned mutant and wild-type acid phosphatase genes such that the concentration reached 200 mg/dl in terms of the dry cell weight. The reaction was conducted at 35° C. for 32 hours while maintaining the pH at 4.0, and the amount of 5′-inosinic acid formed over the course of time was measured. Inosinic acid formed was only 5′-inosinic acid, and the formation of 2′-inosinic acid and 3′-inosinic acid as by-products was not observed at all. The results are shown in FIG. 8.

1. additionTo the solution was added E
2. additionstrain having introduced
3. concentrationthe above-mentioned mutant and wild-type acid phosphatase genes such that the concentration
4. temperaturewhile maintaining the pH at 4.0