(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → HCID 87092300, compound

HRID 278842Notes

Reaction equation

[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4]>C(O)C>[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[CH2:2]([OH:1])[CH3:3]

Participants

Conditions

Temperature: 60 °C

Procedure

Glucose and ethanol were determined by high-performance liquid chromatography (HPLC). Fermentation broth was filtered through a 0.45 μm nylon filter, diluted 10-fold with 0.01N H2SO4, and injected into a Bio-Rad Aminex HPX-87H organic acid column (Waters Chromatography, Milford, Minn.) maintained at 60° C. The components were eluted from the column with 0.01N H2SO4 at a flow rate of 0.5 ml/min. The separated compounds were detected and quantified with a Beckman refractive index detector (Model 156). Known concentrations of compounds were used as standards.

Workup

filtrationFermentation broth was filtered through a 0.45 μm nylon
filtrationfilter
additiondiluted 10-fold with 0.01N H2SO4
washThe components were eluted from the column with 0.01N H2SO4 at a flow rate of 0.5 ml/min

Sources & evidence

ORD record: ord-c2a31a39a3e147f8912adfad9a1afa07
Source dataset: uspto-grants-2002_03
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US06361990B1

Reaction notes

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