反应详情

温度

30 °C

In acetonitrile (14 ml) is dissolved 7β-phenylacetamido-3-hydroxymethyl-3-cephem-4-carboxylic acid triethylamine salt (900 mg), followed by the addition of triethylamine (200 mg), 5-mercapto-1-methyl-1H-tetrazole (464 mg) and N-(methylcarbamoyl)isatin (916 mg). The mixture is stirred at 30° C. overnight. The solvent is then distilled off and the residue is dissolved by the addition of water and ethyl acetate. After separation, the water layer is taken, rinsed once with ethyl acetate and rendered acidic with hydrochloric acid. Following the addition of ethyl acetate, the mixture is stirred for 30 minutes. After separation , the organic layer is taken and rinsed with an aqueous solution of sodium chloride. It is extracted with an aqueous solution of sodium hdyrogen carbonate. The water layer is purified by column chromatography on Sephadex LH-20 . The fractions rich in the contemplated compound are pooled, concentrated, rendered acidic with phosphoric acid and extracted with ethyl acetate. After drying, the extract is concentrated, followed by addition of ether to the residue. The procedure provides 7β-phenylacetamido-3-(1-methyl-1H-tetrazol-5-yl)thiomethyl-3-cephem-4-carboxylic acid. The NMR spectrum of this product is in good agreement with that of the compound obtained in Example 6.

1. distillationThe solvent is then distilled off
2. dissolutionthe residue is dissolved by the addition of water and ethyl acetate
3. customAfter separation
4. washrinsed once with ethyl acetate
5. additionthe addition of ethyl acetate
6. stirringthe mixture is stirred for 30 minutes
7. customAfter separation
8. washrinsed with an aqueous solution of sodium chloride
9. extractionIt is extracted with an aqueous solution of sodium hdyrogen carbonate
10. customThe water layer is purified by column chromatography on Sephadex LH-20
11. concentrationconcentrated
12. extractionextracted with ethyl acetate
13. customAfter drying
14. concentrationthe extract is concentrated
15. additionfollowed by addition of ether to the residue