反应详情

To a stirred solution of 2-amino-5-(4-methoxy-phenyl)-thiophene-3-carboxylic acid (1.0 g, 4.0 mmol) in anhydrous DMF (20 mL) is added (S)-3-amino-azepane-1-carboxylic acid tert-butyl ester (1.03 g, 4.8 mmol), BOP (2.6 g, 6.0 mmol) and NMM (0.6 mL, 5 mmol). The reaction mixture was stirred overnight at room temperature. The solution was diluted with water and EtOAc. The organic layer was separated and set aside. The remaining aqueous layer was extracted with EtOAc (2×) and then the combined organic extracts were pooled and washed with brine. The resultant EtOAc solution was dried over Na2SO4, filtered, and concentrated under vacuum to yield a brown solid. Purification was performed by ISCO MPLC (SiO2, 30-50% EtOAc/hexanes) to give 0.9 g (50%) of an off-white solid. 1H NMR (d6-DMSO δ 7.51, d, 0.5H; δ 7.46, s, 0.5H; δ 7.37, s, 0.5H; δ 7.36, d, 0.5H; δ 7.34, br s, 2H; δ 7.33, d, 2H; δ 6.93, d, 2H; δ 4.11, br s, 1H; δ 3.76, s, 3H; δ 3.61, dq, 1H; δ 3.47, m, 1H; δ 3.11, m, 2H; δ 1.73, m, 3H; δ 1.56, m, 2H; δ 1.42, s, 4.5H; δ 1.38, s+m, 5.5H;), LC/MS (APCI, ES, M+H=446).

1. customThe organic layer was separated
2. extractionThe remaining aqueous layer was extracted with EtOAc (2×)
3. washwashed with brine
4. dry with materialThe resultant EtOAc solution was dried over Na2SO4
5. filtrationfiltered
6. concentrationconcentrated under vacuum
7. customto yield a brown solid
8. customPurification