Sodium Chloride → 7-Amino-2-propylheptanoic acid
Reaction equation
Cl.[NH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH:8]([CH2:12][CH2:13][CH3:14])[C:9]([OH:11])=[O:10].C(=O)([O-])O.[Na+].[Cl-].[Na+].[N-]=[N+]=[N-].[Na+]>O.O1CCOCC1.S([O-])([O-])(=O)=O.[NH4+].[NH4+].P([O-])([O-])([O-])=O>[NH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH:8]([CH2:12][CH2:13][CH3:14])[C:9]([OH:11])=[O:10]Participants
Reactant 4
Product 1
Reagents, catalysts & solvents 4
Procedure
A solution of 7-amino-2-propylheptanoic acid hydrochloride (prepared in EXAMPLE 1, 11.2 mg) in water (0.5 ml) was neutralized with sodium hydrogen carbonate. To the solution was added a solution of m-MBS(15.5 mg) in dioxane (0.5 ml) and allowed to react for 30 minutes at ambient temperature. Then the solution (0.4 ml) was added to a mixture of β-galactosidase (Boeringer Mannheim G.m.b.H., 5 mg/ml suspension in ammonium sulfate solution, 100 μl) in 0.1 M phosphate buffer (pH 7.0, 2 ml) and allowed to react for an hour at ambient temperature. The reaction mixture was applied on a BioGel P-4 (BioRad Corp.) type I column (2.5×25 cm) equilibrated with 0.9% sodium chloride-0.02 M phosphate buffer, and eluted with the same buffer to obtain 5 ml fractions. The β-galactosidase activity of each fractions were measured. Fractions (Nos. 6-8) with high enzyme activity were combined and stored in a 0.1% sodium azide (preservative) and 0.1% BSA (stabilizer) solution.
Workup
- customto react for 30 minutes at ambient temperature
- customto react for an hour at ambient temperature
- washeluted with the same buffer
- customto obtain 5 ml fractions
Sources & evidence
- ORD record
- ord-e03a38e9f5974550a4ce1f1cfdd1687d
- Source dataset
- uspto-grants-1984_04
- Patent number
- US04443365
Reaction notes
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