反应详情

1.1 g (0.00464 mole) of 4-amino-7-hydroxy-2-(methoxymethoxymethyl)pteridine is added to a solution of 1.5 g (0.0267 mole) of potassium hydroxide in 20 ml of water. The mixture is refluxed for 8 hours. After cooling, the reaction mixture is acidified with 10% hydrochloric acid to pH 5.5. The precipitate formed is isolated by filtration and washed with water. It is extracted with a mixture of 50 ml of ethanol and 60 ml of N,N-dimethylformamide under reflux. This extract, concentrated under reduced pressure, is recrystallized from a mixture of ethanol and N,N-dimethylformamide. Yld: 0.2 g (18%), m.p. 224°-226° C. (ethanol).

1. temperatureThe mixture is refluxed for 8 hours
2. temperatureAfter cooling
3. customThe precipitate formed
4. customis isolated by filtration
5. washwashed with water
6. extractionIt is extracted with a mixture of 50 ml of ethanol and 60 ml of N,N-dimethylformamide
7. temperatureunder reflux
8. concentrationThis extract, concentrated under reduced pressure
9. customis recrystallized from a mixture of ethanol and N,N-dimethylformamide