反应详情

To 400 mg 1-[(5-Chloro-pyridin-2-ylcarbamoyl)-methyl]-5-[2-(2-methoxy-ethoxy)-ethoxymethyl]-1H-pyrazole-3-carboxylic acid in 5 ml DCM and 0.3 ml NEt3, 217 mg 1-Isopropyl-piperidin-4-ylamine hydrochloride and 250 mg BOP-Cl were added at RT and the rmixture was stirred for 16 h. The mixture was concentrated under reduced pressure and the residue was purified by preparative HPLC (C18 reverse phase column, elution with a H2O/MeCN gradient with 0.1% TFA). The fractions containing the product were evaporated and lyophilized to yield a white solid.

1. concentrationThe mixture was concentrated under reduced pressure
2. customthe residue was purified by preparative HPLC (
3. washC18 reverse phase column, elution with a H2O/MeCN gradient with 0.1% TFA)
4. additionThe fractions containing the product
5. customwere evaporated
6. customto yield a white solid