Silane → Nonanal
Reaction equation
CCC[CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][O:13]S([O-])(=O)=O.[Na+].[SiH4]>>[CH:12](=[O:13])[CH2:11][CH2:10][CH2:9][CH2:8][CH2:7][CH2:6][CH2:5][CH3:4]Participants
3
Reactant 2
Product 1
Procedure
Targets are complementary oligonulceotides labeled with fluorescent dye Cy3. Slides were hybridized with targets in hybridization buffer (2×SSC, 0.1% SDS and complementary target at 100 nM) at room temperature for 1 hr and washed in 2×SSC, 0.1% SDS for 5 min., 0.1×SSC, 0.1% SDS for 10 min., and finally in 0.1×SSC for 1 min. All washings were carried out at room temperature. Results show that the aldehyde slides prepared from photoactivatable silane gave much higher hybridization signal than non-aldehyde slides.
Workup
- washwashed in 2×SSC, 0.1% SDS for 5 min.
- waitfinally in 0.1×SSC for 1 min
- customwere carried out at room temperature
- customResults
Sources & evidence
- ORD record
- ord-89c4572ca97d435ab49ba8ac02e6b6f6
- Source dataset
- uspto-grants-2007_02
- Patent number
- US07176297B2
Reaction notes
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