反应详情

The compound (120 mg) obtained in Example 47-3 was dissolved in methanol (5.0 ml) and then added with the compound (58.0 mg) obtained in Example 90-1 and sodium cyanoborohydride (18.0 mg). Then, the solution was adjusted to pH 5 with acetic acid and then stirred at room temperature for 2 days. After completion of the reaction, a 1 mol/l sodium hydroxide aqueous solution was added to the reaction solution, followed by separation/extraction with chloroform. The organic layer was dried with anhydrous sodium sulfate and the solvent was then distilled off. The residue was purified through silica gel column chromatography (chloroform/methanol) and then treated with hydrochloric acid, thereby obtaining hydrochloride (107 mg) of the subject compound as a white solid.

1. customAfter completion of the reaction
2. extractionfollowed by separation/extraction with chloroform
3. dry with materialThe organic layer was dried with anhydrous sodium sulfate
4. distillationthe solvent was then distilled off
5. customThe residue was purified through silica gel column chromatography (chloroform/methanol)
6. additiontreated with hydrochloric acid