HRID35800

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：O=[N+]([O-])c1ccccc1O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

HCID96647

2-Nitrophenyl-beta-D-galactopyranoside

C12H15NO8

2 次

结构：OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O

HCID440995

(+)-Lactose

C12H22O11

PRODUCTS

生成物

HCID798

Indole

C8H7N

PROCEDURE

实验过程

Principle of Step 1: ONPG substituting for lactose is metabolized with β-galactosidase. At 44.5° C. E. coli grow and utilize ONPG and produce indole. β-galactoside turns the medium yellow. In the yellow colored medium, both fecal coliforms and E. coli are present. In samples which do not have a yellow color, there are no fecal coliforms and E. coli.

WORKUP

后处理

customAt 44.5° C