HRID359340

反应详情

EQUATION

反应方程式

REACTANTS

反应物

HCID6116

Calcium acetate

C4H6CaO4

结构：Cc1ccc(S(=O)(=O)NCC(=O)N2CCC[C@H]2C(=O)N[C@@H](CCCNC(=N)N)C(=O)Nc2ccc([N+](=O)[O-])cc2)cc1

HCID178427468

未命名化合物

PRODUCTS

生成物

HCID7475

4-Nitrobenzenamine

C6H6N2O2

结构：Cc1ccc(S(=O)(=O)NCC(=O)N2CCC[C@H]2C(=O)N[C@@H](CCCNC(=N)N)C(=O)O)cc1

HCID178427469

未命名化合物

PROCEDURE

实验过程

After precisely 4 minutes, 150 μl. of starting reagent, consisting of 1.1 mmole/litre Chromozym® TH (Tos-Gly-ProArg-pNA) and 100 mmole/litre calcium acetate, are added thereto and at 405 nm there is determined in a photometer the time until a definite amount of substrate is reacted by newly formed thrombin to give Tos-Gly-Pro-Arg and p-nitroaniline (pNA). The amount of reacted substrate, which is measured, is defined by a threshold extinction value (for example ΔE=0.2).

WORKUP

后处理

additionare added
customis reacted by newly formed thrombin

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-6e2ad97def424f37a65c9f83ecd56811
来源数据集: uspto-grants-1991_03
DOI: 10.6084/m9.figshare.5104873.v1
专利: US05001069
来源说明: uspto-grants-1991_03

反应 SMILES

[NH:1]([S:33]([C:36]1[CH:42]=[CH:41][C:39]([CH3:40])=[CH:38][CH:37]=1)(=[O:35])=[O:34])[CH2:2][C:3]([N:5]1[CH2:32][CH2:31][CH2:30][C@H:6]1[C:7]([NH:9][C@H:10]([C:18]([NH:20][C:21]1[CH:29]=[CH:28][C:24]([N+:25]([O-:27])=[O:26])=[CH:23][CH:22]=1)=[O:19])[CH2:11][CH2:12][CH2:13][NH:14][C:15](=[NH:17])[NH2:16])=[O:8])=[O:4].C([O-])(=[O:45])C.[Ca+2].C([O-])(=O)C>>[NH:1]([S:33]([C:36]1[CH:37]=[CH:38][C:39]([CH3:40])=[CH:41][CH:42]=1)(=[O:35])=[O:34])[CH2:2][C:3]([N:5]1[CH2:32][CH2:31][CH2:30][C@H:6]1[C:7]([NH:9][C@H:10]([C:18]([OH:45])=[O:19])[CH2:11][CH2:12][CH2:13][NH:14][C:15](=[NH:17])[NH2:16])=[O:8])=[O:4].[N+:25]([C:24]1[CH:28]=[CH:29][C:21]([NH2:20])=[CH:22][CH:23]=1)([O-:27])=[O:26]