反应详情

Benzylchloroformate (161 mL, 1075 mmol, Aldrich, 95%) was added slowly through an addition funnel at room temperature with vigorous stirring to a solution of the sulfate salt of 2-methyl-2-thiopseudourea (74.6 g, 525 mmol) in 1N sodium hydroxide solution (1050 mL, 1050 mmol) in a 5 L three-necked round bottom flask, equipped with a 250 mL addition funnel. After the addition, stirring was continued and the reaction was monitored by TLC. The reaction was finished in ca. 20 hours. After all mono-CBZ (benzyloxy carbonyl) intermediate disappeared, the reaction mixture was transferred to a 4 L separatory funnel. The aqueous layer was separated and extracted with hexanes (1.28 L). The extracts were combined with the methylene chloride layer. The combined organic layer was washed with 553 mL of NaH2PO4 (5.7%, 533 mL, wash pH ~5) and brine (2×533 mL). It was dried over sodium sulfate, filtered and concentrated on a rotovap. The resulting residual oil was dried on a high vacuum pump to a constant weight. The remaining benzyl chloroformate, benzyl chloride and possibly benzyl alcohol were further removed by a lyophilizer. The resulting thick oil crystallized on standing in about six hours. The solid was broken up and the drying was continued to a constant weight in two days. The crude title compound thus obtained (182 g, 97% yield), was pure enough by 1H NMR and TLC to be used for the next reaction without any further purification.

1. customequipped with a 250 mL addition funnel
2. additionAfter the addition
3. customthe reaction mixture was transferred to a 4 L separatory funnel
4. customThe aqueous layer was separated
5. extractionextracted with hexanes (1.28 L)
6. washThe combined organic layer was washed with 553 mL of NaH2PO4 (5.7%, 533 mL, wash pH ~5) and brine (2×533 mL)
7. dry with materialIt was dried over sodium sulfate
8. filtrationfiltered
9. concentrationconcentrated on a rotovap
10. customThe resulting residual oil was dried on a high vacuum pump to a constant weight
11. customThe remaining benzyl chloroformate, benzyl chloride and possibly benzyl alcohol were further removed by a lyophilizer
12. customThe resulting thick oil crystallized
13. waiton standing in about six hours
14. waitthe drying was continued to a constant weight in two days