5'-Atp → HCID 178518213, compound

HRID 363285Notes

Reaction equation

HRID 363285 reaction equation
[C@@H:1]1([N:10]2[C:19]3[N:18]=[CH:17][N:16]=[C:14]([OH:15])[C:13]=3[N:12]=[CH:11]2)[O:9][C@H:6]([CH2:7][OH:8])[C@@H:4]([OH:5])[C@H:2]1[OH:3].[P:20]([O:32][CH2:33][C@H:34]1[O:38][C@@H:37]([N:39]2[C:48]3[N:47]=[CH:46][N:45]=[C:43](N)[C:42]=3[N:41]=[CH:40]2)[C@H:36]([OH:49])[C@@H:35]1[OH:50])([O:23]P(OP(O)(O)=O)(O)=O)(=[O:22])[OH:21].[OH-].[Na+].P(O)(O)([O-])=[O:54].[K+]>>[C@@H:1]1([N:10]2[C:19]3[N:18]=[CH:17][N:16]=[C:14]([OH:15])[C:13]=3[N:12]=[CH:11]2)[O:9][C@H:6]([CH2:7][OH:8])[C@@H:4]([OH:5])[C@H:2]1[OH:3].[CH:46]1[NH:47][C:48]2[N:39]([C@@H:37]3[O:38][C@H:34]([CH2:33][O:32][P:20]([OH:21])([OH:23])=[O:22])[C@@H:35]([OH:50])[C@H:36]3[OH:49])[CH:40]=[N:41][C:42]=2[C:43](=[O:54])[N:45]=1

Participants

6

Reactant 4

HCID 59575'-AtpHCID 14798Sodium HydroxideHCID 516951Monobasic potassium phosphateHCID 178521066(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

Product 2

HCID 178518213HCID 178518213, compoundHCID 178521066(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

Procedure

After completion of fermentation, about 30 g/l of inosine was accumulated in the medium obained in (8) above. As indicated in Table 9, 20 ml of a reaction mixture containing this medium was prepared. In a manner similar to (9), while keeping at 32° C. with vigorously stirring and also keeping pH at 7.2 with 4N NaOH, a reaction was carried out for 13 hours. During the course of reaction, monopotassium phosphate was added as in (9). From 23.5 g/l of initial inosine, 46 g/l of 5'-IMP was produced and accumulated. Also in this case, its molar conversion rate was 90% or more, as in (9).

Workup

  1. additioncontaining this medium
  2. customwas prepared
  3. customwhile keeping at 32° C.

Sources & evidence

ORD record
ord-c7ee8822f04e4e4f8a298542ae390a4c
Source dataset
uspto-grants-1999_02
Patent number
US05874272

Reaction notes

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