Ampicillin → Maleic Acid

Participants

Reaction Steps

The ampicillin-resistant transformant was selected by measuring its enzyme activity of isomerizing maleic acid. The ampicillin-resistant transformant was inoculated via a loop of platinum into 3 ml maleic acid medium (Table 1) containing 100 γ ampicillin and cultured overnight, and the microorganism was recovered by centrifuging 1.5 ml of the culture. The recovered microorganism was suspended in 1 ml of 20% maleic acid substrate solution (Table 2) and reacted at 30° C. overnight. This substrate solution contained aspartase prepared from E. coli so that fumaric acid formed by isomerization of maleic acid was converted into L-aspartic acid. After overnight reaction, a part of the reaction solution was diluted 400-fold with distilled water and measured for its formed L-aspartic acid by HPLC analysis. The conditions for HPLC analysis were as described in item (2) in Example 1.

Provenance & Evidence