L-Aspartic Acid → L-Alanine
Reaction equation
[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][C:4]([OH:6])=[O:5]>O>[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH3:3].[NH2:1][C@H:2]([C:7]([OH:9])=[O:8])[CH2:3][C:4]([OH:6])=[O:5]Participants
Reactant 1
Product 2
Reagents, catalysts & solvents 1
Conditions
- Temperature
- 25 °C
Procedure
A water-insoluble tannin preparation [i.e. chinese gallotannin covalently bound to cellulose through the linkage --CH2 --CH(OH)CH2NH--(CH2)6 --NHCH2CH(OH)CH2 --] is prepared in the same manner as described in Example 1-(2). 4 ml of an aqueous solution of L-aspartic acid β-decarboxylase (total activity: 5,640 μ moles/hr) obtained from Pseudomonas dacunhae are added to one g (wet form) of the water-insoluble tannin preparation, and the mixture is shaken at 25° C for 30 minutes. Then, the precipitates are collected by filtration and washed with water. 0.95 g (wet form) of an immobilized L-aspartic acid β-decarboxylase preparation [i.e. L-aspartic acid β-decarboxylase adsorbed on the water-insoluble tannin preparation] are thereby obtained. It shows a L-aspartic acid β-decarboxylase activity of 180 μ moles/hr. [Aspartic acid β-decarboxylase activity is indicated in terms of micromoles of L-alanine which are produced by reaction with 0.2 M L-aspartic acid at 37° C at pH 5.5].
Workup
- customis prepared in the same manner
- filtrationThen, the precipitates are collected by filtration
- washwashed with water
- custom0.95 g (wet form) of an immobilized L-aspartic acid β-decarboxylase preparation [i.e. L-aspartic acid β-decarboxylase
- customare thereby obtained
Sources & evidence
- ORD record
- ord-d86876e595df43ed926136e540c122bd
- Source dataset
- uspto-grants-1978_05
- Patent number
- US04090919
Reaction notes
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