L-Aspartic Acid → L-Alanine

Participants

Reaction Conditions

Reaction Steps

A water-insoluble tannin preparation [i.e. chinese gallotannin covalently bound to cellulose through the linkage --CH2 --CH(OH)CH2NH--(CH2)6 --NHCH2CH(OH)CH2 --] is prepared in the same manner as described in Example 1-(2). 4 ml of an aqueous solution of L-aspartic acid β-decarboxylase (total activity: 5,640 μ moles/hr) obtained from Pseudomonas dacunhae are added to one g (wet form) of the water-insoluble tannin preparation, and the mixture is shaken at 25° C for 30 minutes. Then, the precipitates are collected by filtration and washed with water. 0.95 g (wet form) of an immobilized L-aspartic acid β-decarboxylase preparation [i.e. L-aspartic acid β-decarboxylase adsorbed on the water-insoluble tannin preparation] are thereby obtained. It shows a L-aspartic acid β-decarboxylase activity of 180 μ moles/hr. [Aspartic acid β-decarboxylase activity is indicated in terms of micromoles of L-alanine which are produced by reaction with 0.2 M L-aspartic acid at 37° C at pH 5.5].

Provenance & Evidence