Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Methane | HCID 297 | — |
| Reactant | Sucrose | HCID 5988 | — |
| Product | Trehalose | HCID 7427 | — |
Reaction Steps
Incubation of Brevibacterium lactofermentum ATCC 13869 was effected in the same manner as in Example 1, except that sucrose was used as the carbon source. One liter of the culture obtained was sterilized, then treated with an anion exchange resin AMBERLITE IR-4B and a cation exchange resin AMBERLITE IR-120 and decolored with active charcoal. Next, the decolored liquid was concentrated to about 25 ml, and 75 ml of ethanol was added thereto and left to cool to 5° C. to obtain 8.5 g of trehalose crystals having a purity of 99%.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US05484714 |
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| Source dataset | uspto-grants-1996_01 |
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| ORD ID | ord-b2918bc31bf04b3691d63fbb0871c0b9 |
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| Source note | uspto-grants-1996_01 |
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