HRID375061

反应详情

EQUATION

反应方程式

REACTANTS

反应物

HCID297

Methane

CH4

结构：OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@@H]1O

HCID5988

Sucrose

C12H22O11

PRODUCTS

生成物

结构：OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

HCID7427

D-Trehalose

C12H22O11

CONDITIONS

反应条件

温度: 5 °C

PROCEDURE

实验过程

Incubation of Brevibacterium lactofermentum ATCC 13869 was effected in the same manner as in Example 1, except that sucrose was used as the carbon source. One liter of the culture obtained was sterilized, then treated with an anion exchange resin AMBERLITE IR-4B and a cation exchange resin AMBERLITE IR-120 and decolored with active charcoal. Next, the decolored liquid was concentrated to about 25 ml, and 75 ml of ethanol was added thereto and left to cool to 5° C. to obtain 8.5 g of trehalose crystals having a purity of 99%.

WORKUP

后处理

customOne liter of the culture obtained
additiontreated with an anion exchange resin AMBERLITE IR-4B
concentrationNext, the decolored liquid was concentrated to about 25 ml, and 75 ml of ethanol
additionwas added
waitleft