反应详情

温度

-10 °C

Phosphorus pentachloride (2.15g, 10.32mmol) in dichloromethane (108ml) was added to 4-methoxybenzyl (6R,7R)-7-phenylacetamido-3-[(RS)-tetrahydrofuran-2-yl]ceph-3-em-4-carboxylate (3.40g, 6.69mmol) and N-methylmorpholine (1.50 ml, 13.64mmol) in dichloromethane (30ml) at -25° C. The reaction was stirred at -10±5° C. for 45min., then methanol (14ml) was added, and stirring continued for 45min. at room temperature. Water (27ml) was then added, and the mixture vigorously stirred for a further 1h. The dichloromethane was evaporated in vacuo, the aqueous residue washed with diethyl ether, then adjusted to pH7 with ammonium hydroxide in the presence of ethyl acetate. The mixture was extracted with ethyl acetate (x2), dried and concentrated in vacuo. The residue was purified by chromatography on silica gel eluting with 50, 70, 80% ethyl acetate in hexane yielding 4-methoxybenzyl (6R,7R)-7-amino-3-[(S)-tetrahydrofuran-2-yl]ceph-3-em-4-carboxylate (980mg, 38%) as a yellow foam; vmax (CH2Cl2) 1777, 1721, 1613, 1516, 1152cm-1 ; δH (CDCl3, 250MHz) 1.53-1.71 (1H, m), 1.84-2.02 (4H, m, 2 exch.), 2.25-2.40 (1H, m), 3.31 and 3.60 (2H, ABq, J 18.5Hz), 3.78-3.98 (2H, m), 3.81 (3H, s), 4.72 (1H, d, J 5.0Hz), 4.86-4.93 (2H, m), 5.19 (2H, s), 6.88 (2H, d, J 8.6Hz), 7.33 (2H, d, J 8.6Hz). [Mass spectrum: M+ (390)].

1. stirringstirring
2. waitcontinued for 45min. at room temperature
3. stirringthe mixture vigorously stirred for a further 1h
4. customThe dichloromethane was evaporated in vacuo
5. washthe aqueous residue washed with diethyl ether
6. extractionThe mixture was extracted with ethyl acetate (x2)
7. customdried
8. concentrationconcentrated in vacuo
9. customThe residue was purified by chromatography on silica gel eluting with 50, 70, 80% ethyl acetate in hexane