反应详情

To a solution of 4-morpholinecarbonylphenylalanine (Mu-PheOH, 0.14 g, 0.48 mmol, prepared according to the method described in Esser, R. et.al., Arthritis & Rheumatism (1994), 37, 236) in THF (10 mL)) at -10° C. were added 4-methylmorpholine (0.11 mL, 0.96 mmol), followed by isobutyl chloroformate (64 μL, 0.48 mmol). After 5 minute activation, 2-phenethyl-1-phenylsulfonylethylenediamine hydrochloride (0.15 g, 0.44 mmol, prepared by conversion of the N-phenylsulfonylhomophenylalanine to the amide via CDl/NH3 /THF reaction, then by reduction of the carbonyl amide to its corresponding amine with lithium aluminium hydride, followed by treatment with HCl in dioxane) was added. After 1 hour, the reaction mixture was diluted with CH2Cl2 (50 mL) and washed with 1 M HCl (50 mL) followed by saturated aqueous NaHCO3 (50 mL), dried over MgSO4, filtered, and the solvent was removed under reduced pressure. The residue was diluted with CH2Cl2 (5 mL) and precipated from hexane (200 mL), filtered, and dried in vacuo yielding 0.22 g (89%) of the desired product.

1. additionwas added
2. washwashed with 1 M HCl (50 mL)
3. dry with materialdried over MgSO4
4. filtrationfiltered
5. customthe solvent was removed under reduced pressure
6. additionThe residue was diluted with CH2Cl2 (5 mL)
7. filtrationfiltered
8. customdried in vacuo