HCID 178513440, compound → HCID 178460549, compound

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Reaction Steps

By spectrophotometric analysis, the elutions in 100 ul of 10 mM Tris HCl at pH 9.5 yielded 30 μg (A260/A280 of 1.78) of DNA for the bimodal urea-histidine -propionate IE particles and less than 2 μg of DNA for the urea-histidine IE particles. No DNA was detected on analysis of the eluent from the urea-histidine IE particles, by gel electrophoresis. The results above indicate that the addition of propionate to the urea-histidine particles lowered the needed concentration of counter-ion (chloride) required for elution of the DNA at pH 4.8, 7.3 and 9.5.

Provenance & Evidence