HCID 178513440, compound → HCID 178460549, compound

HRID 382771Notes

Reaction equation

HRID 382771 reaction equation
[NH2:1][C:2]([NH2:4])=[O:3].[NH2:5][C@H:6]([C:13]([OH:15])=[O:14])[CH2:7][C:8]1[N:12]=[CH:11][NH:10][CH:9]=1>C(O)C(N)(CO)CO.Cl>[NH2:1][C:2]([NH2:4])=[O:3].[C:13]([OH:15])(=[O:14])[CH2:6][CH3:7].[NH2:5][C@H:6]([C:13]([OH:15])=[O:14])[CH2:7][C:8]1[N:12]=[CH:11][NH:10][CH:9]=1

Participants

3

Reactant 1

HCID 178513440HCID 178513440, compound

Product 1

HCID 178460549HCID 178460549, compound

Reagents, catalysts & solvents 1

HCID 93573Tromethamine hydrochlorideSolvent

Procedure

By spectrophotometric analysis, the elutions in 100 ul of 10 mM Tris HCl at pH 9.5 yielded 30 μg (A260/A280 of 1.78) of DNA for the bimodal urea-histidine -propionate IE particles and less than 2 μg of DNA for the urea-histidine IE particles. No DNA was detected on analysis of the eluent from the urea-histidine IE particles, by gel electrophoresis. The results above indicate that the addition of propionate to the urea-histidine particles lowered the needed concentration of counter-ion (chloride) required for elution of the DNA at pH 4.8, 7.3 and 9.5.

Sources & evidence

ORD record
ord-296e00341b9b41d8858463767ae8b36d
Source dataset
uspto-grants-2001_10
Patent number
US06310199B1

Reaction notes

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