Tromethamine hydrochloride → HCID 178460548, compound
Reaction equation
[NH2:1][C:2]([NH2:4])=[O:3].[NH2:5][C@H:6]([C:13]([OH:15])=[O:14])[CH2:7][C:8]1[N:12]=[CH:11][NH:10][CH:9]=1.C(O)C(N)(CO)CO.Cl>>[NH2:1][C:2]([NH2:4])=[O:3].[NH2:5][C@H:6]([C:13]([OH:15])=[O:14])[CH2:7][C:8]1[N:12]=[CH:11][NH:10][CH:9]=1.[C:13]([O-:15])(=[O:14])[CH2:6][CH3:7]Participants
3
Procedure
By spectrophotometric analysis, the elutions in 100 ul of 10 mM Tris HCl at pH 9.5 yielded 30 μg (A260/A280 of 1.78) of DNA for the bimodal urea-histidine-propionate IE particles and less than 2 μg of DNA for the urea-histidine IE particles. No DNA was detected on analysis of the eluent from the urea-histidine IE particles, by gel electrophoresis.
Sources & evidence
- ORD record
- ord-5a0a98d368ce44a9afb84ac5edebf7dc
- Source dataset
- uspto-grants-2004_10
- Patent number
- US06806362B2
Reaction notes
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