HCID 86588919, compound → HCID 57196060, compound

HRID 399581Notes

Reaction equation

[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[CH:27]=[C:26]([C:28]#[C:29][CH2:30][O:31][CH2:32][CH2:33][O:34][CH2:35][CH2:36][NH:37]C(=O)C(F)(F)F)[C:24]([NH2:25])=[N:23][C:21]2=[O:22])[CH2:17][CH2:16]1)([O:4][P:5]([O:8][P:9]([OH:12])([OH:11])=[O:10])([OH:7])=[O:6])(=[O:3])[OH:2]>[NH4+].[OH-]>[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[CH:27]=[C:26]([C:28]#[C:29][CH2:30][O:31][CH2:32][CH2:33][O:34][CH2:35][CH2:36][NH2:37])[C:24]([NH2:25])=[N:23][C:21]2=[O:22])[CH2:17][CH2:16]1)([O:4][P:5]([O:8][P:9]([OH:11])([OH:12])=[O:10])([OH:7])=[O:6])(=[O:2])[OH:3]

Participants

Conditions

Temperature: 48 °C

Procedure

The purified protected triphosphate 22 was taken up in concentrated aqueous NH4OH (2 mL) and stirred for 3 h at approximately 48° C. The reaction mixture was concentrated to give compound 23 which was formulated with 0.1M TEAB pH 7.0 to a concentration of 3.5 mM. The concentration and purity of the formulated bulk were confirmed by UV/Vis spectroscopy and analytical ion pair HPLC as described above, respectively. See FIG. 12.

Workup

concentrationThe reaction mixture was concentrated

Sources & evidence

ORD record: ord-fdc08aa87e3042f68a4e80957c9d2d0a
Source dataset: uspto-grants-1998_10
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05821356

Reaction notes

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