反应详情

To a solution of tert-butyl 4-((1R,3aS,5aR,5bR,7aR,11aS,11bR,13aR,13bR)-5a,5b,8,8,11a-pentamethyl-3a-((2-(piperidin-1-yl)ethylamino)methyl)-1-(prop-1-en-2-yl)-2,3,3a,4,5,5a,5b,6,7,7a,8,11,11a,11b,12,13,13a,13b-octadecahydro-1H-cyclopenta[a]chrysen-9-yl)benzoate (119 mg, 0.167 mmol) in DCM (1 ml) was added TFA (0.4 ml, 5.19 mmol). The mixture was stirred at rt for 6 h then was concentrated under reduced pressure. The residue was dissolved in dioxane and MeOH and was purified by prep HPLC. The fractions containing the expected product were combined and concentrated under reduced pressure. The residue was dissolved in dioxane and methanol and the mixture was heated with a heat gun to reflux. Water was slowly added until the mixture was slightly cloudy. The mixture was allowed to cool to rt and then was refrigerated overnight. The solids that formed were collected by filtration and were washed with water to afford the title compound as a light-yellow solid (57 mg, 0.087 mmol, 52.1% yield). LCMS: m/e 655.6 (M+H)+, 2.28 min (method 6). 1H NMR (400 MHz, Acetic Acid d4) δ ppm 7.99 (d, J=8.28 Hz, 2H), 7.25 (d, J=8.28 Hz, 2H), 5.32 (d, J=4.52 Hz, 1H), 4.75 (s, 1H), 4.63 (s, 1H), 3.72 (s, 2H), 3.56-3.66 (m, 2H), 3.33 (d, J=12.80 Hz, 1H), 2.98 (d, J=12.80 Hz, 1H), 2.48 (br. s., 1H), 1.71 (s, 3H), 1.14 (s, 3H), 1.09-2.22 (m, 32H), 1.05 (s, 3H), 1.03 (s, 3H), 0.97 (s, 3H), 0.96 (s, 3H).

1. concentrationthen was concentrated under reduced pressure
2. dissolutionThe residue was dissolved in dioxane
3. customMeOH and was purified by prep HPLC
4. additionThe fractions containing the expected product
5. concentrationconcentrated under reduced pressure
6. dissolutionThe residue was dissolved in dioxane
7. temperaturemethanol and the mixture was heated with a heat gun
8. temperatureto reflux
9. additionWater was slowly added until the mixture
10. temperatureto cool to rt
11. waitwas refrigerated overnight
12. customThe solids that formed
13. filtrationwere collected by filtration
14. washwere washed with water