Glycine → 4-Nitrophenol

HRID 411106Notes

Reaction equation

HRID 411106 reaction equation
[CH:1]1[C:6]([N+:7]([O-:9])=[O:8])=[CH:5][CH:4]=[C:3]([O:10][C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[CH:2]=1.C(O)[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O.C(O)(=O)C.[Na].NCC(O)=O>>[CH:5]1[C:6]([N+:7]([O-:9])=[O:8])=[CH:1][CH:2]=[C:3]([OH:10])[CH:4]=1

Participants

5

Reactant 4

HCID 750GlycineHCID 7027delta-GluconolactoneHCID 1651254-Nitrophenyl 4-O-beta-D-glucopyranosyl-beta-D-glucopyranosideHCID 24188569126-96-5

Product 1

HCID 9804-Nitrophenol

Procedure

To determine the influence of the temperature on the enzyme activity 25 μl enzyme solution was added to 225 μl of a solution containing 0.1% p-nitrophenyl-β-D-cellobioside and 25 mM gluconolactone in a 50 mM sodium acetic acid buffer at pH 5.0. Following an incubation of 15 minutes at 30, 40, 50, 60 or 70° C. the enzyme activity was stopped by adding 750 μl of a 0.5 M glycine buffer pH 9.0. The concentration of p-nitrophenol formed was determined spectrophotometrically at 400 nm.

Workup

  1. customan incubation of 15 minutes
  2. customat 30, 40, 50, 60 or 70° C.

Sources & evidence

ORD record
ord-15f32c2071fe4df7bfa2b8a7cea54026
Source dataset
uspto-grants-2003_05
Patent number
US06558937B1

Reaction notes

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