Glycine → 4-Nitrophenol

Participants

Reaction Steps

To determine the influence of the temperature on the enzyme activity 25 μl enzyme solution was added to 225 μl of a solution containing 0.1% p-nitrophenyl-β-D-cellobioside and 25 mM gluconolactone in a 50 mM sodium acetic acid buffer at pH 5.0. Following an incubation of 15 minutes at 30, 40, 50, 60 or 70° C. the enzyme activity was stopped by adding 750 μl of a 0.5 M glycine buffer pH 9.0. The concentration of p-nitrophenol formed was determined spectrophotometrically at 400 nm.

Provenance & Evidence