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Phosphate buffered saline solution was prepared in the following manner. An 18.8 g. portion of anhydrous dibasic potassium phosphate was dissolved in 140 ml. distilled water preheated to 45° C. To this solution were added 3.3 g. monobasic sodium phosphate, and it was stirred until dissolved. Then 45 g. sodium chloride were added with stirring. After the sodium chloride was dissolved, the solution was brought to 200 ml. with distilled water. A 40 ml. portion of the above solution was then mixed with 960 ml. distilled water to form a 0.01M phosphate buffered saline solution (PBS) at pH 7.5. It was then stored at 4° C. Larger quantities were also prepared using the above proportions.

1. customPhosphate buffered saline solution was prepared in the following manner
2. custompreheated to 45° C
3. additionTo this solution were added 3.3 g
4. dissolutionuntil dissolved
5. additionportion of the above solution was then mixed with 960 ml