反应详情

To a solution of rac-(2′S,3′R,4′S,5′R)-6-chloro-4′-(3-chloro-2-fluoro-phenyl)-2′-(2,2-dimethyl-propyl)-2-oxo-1,2-dihydro-spiro[indole-3,3′-pyrrolidine]-5′-carboxylic acid trifluoroacetic acid prepared in Example 4 (0.3 g, 0.52 mmol) in dichloromethane (9 mL) was added diisopropylethylamine (0.34 g, 2.6 mmol), diphenylphosphinic chloride (Aldrich) (0.24 g, 1 mmol) respectively. The mixture was stirred at room temperature for 8 min, then 6-[2-(tert-butyl-dimethyl-silanyloxy)-ethoxy]-4-methoxy-pyridin-3-ylamine prepared in Example 191 (0.23 g, 0.78 mmol) was added. The reaction mixture was stirred at room temperature for 48 h. The mixture was concentrated. The residue was dissolved into tetrahydrofuran (3 mL), and an aqueous solution (1N) of HCl (1 mL) was added. The reaction mixture was stirred at room temperature for 1 h, then concentrated. The residue was partitioned between ethyl acetate and aqueous saturated NaHCO3 solution. The organic layer was separated, and aqueous layer was extracted with ethyl acetate twice. The combined organic extract was washed with water, brine, dried over Na2SO4, then concentrated. The residue was purified by chromatography (0-15% MeOH in CH2Cl2) to give rac-(2′S,3′R,4′S,5′R)-6-chloro-4′-(3-chloro-2-fluoro-phenyl)-2′-(2,2-dimethyl-propyl)-2-oxo-1,2-dihydro-spiro[indole-3,3′-pyrrolidine]-5′-carboxylic acid [6-(2-hydroxy-ethoxy)-4-methoxy-pyridin-3-yl]-amide as a white solid (0.15 g, 46%).

1. stirringThe reaction mixture was stirred at room temperature for 48 h
2. concentrationThe mixture was concentrated
3. dissolutionThe residue was dissolved into tetrahydrofuran (3 mL)
4. additionan aqueous solution (1N) of HCl (1 mL) was added
5. stirringThe reaction mixture was stirred at room temperature for 1 h
6. concentrationconcentrated
7. customThe residue was partitioned between ethyl acetate and aqueous saturated NaHCO3 solution
8. customThe organic layer was separated
9. extractionaqueous layer was extracted with ethyl acetate twice
10. extractionThe combined organic extract
11. washwas washed with water, brine
12. dry with materialdried over Na2SO4
13. concentrationconcentrated
14. customThe residue was purified by chromatography (0-15% MeOH in CH2Cl2)