反应详情

温度

-24 °C

At 2° C. a mantled reactor with mechanical stirrer, dropping funnel and inner thermometer was charged with DMA (640 ml), triethylamine (224 ml, 161.3 g, 1.59 mol) and 5-Amino-2,4,6-triiodo-isophthalic acid dichloride (320 g, 537 mmol). The mixture was stirred and cooled to −24° C. A solution of 1-amino-2,3-dihydroxypropane (49.92 g, 548 mmol) in DMA (160 ml) was added slowly to keep the inner temperature below −19° C. The mixture was stirred at a temperature gradient from −24° C. to 0° C. in 24 h. A solution of serinol (60 g, 658 mmol) in DMA (160 ml) was added slowly and the mixture was stirred at a temperature gradient from 0° C. to 40° C. in 20 h. The mixture was stirred at 22° C. for 1 day and precipitated triethylamine hydrochloride was filtered off. Evaporation at 60° C./25 mbar left a viscous liquid residue (586 g) which was diluted with water (350 ml). Salts and excess amines were removed by treatment with ion exchangers Amberlite200C (143 ml), IRA67 (148 ml) and IRA900 (56 ml) followed by filtration. The ion exchangers were washed with water (2×400 ml). Some seeding crystals were added to the combined aqueous phase and the solution was stirred slowly at 22° C. for 9 days. The precipitate was isolated by filtration. The filter cake was re-suspended in water (240 ml) and stirred for 1 day. The suspension was filtered and the filter cake was dried in air (216 g, 57% yield, 88.6% HPLC purity). The crude product was purified by preparative HPLC (column: selfpacked Luna C18, 10 μm 250×100 mm, solvents: A=water and B=acetonitrile; gradient 5-10% B over 10 min, hold 10 min; flow 350 ml/min, UV detection at 244 nm and 254 nm). Relevant fractions were combined and freeze dried to yield 5-amino-N1-(1,3-dihydroxypropan-2-yl)-N3-(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamide.

1. customAt 2° C. a mantled reactor with mechanical stirrer, dropping
2. customthe inner temperature below −19° C
3. stirringThe mixture was stirred at a temperature gradient from −24° C. to 0° C. in 24 h
4. stirringthe mixture was stirred at a temperature gradient from 0° C. to 40° C. in 20 h
5. stirringThe mixture was stirred at 22° C. for 1 day
6. customprecipitated triethylamine hydrochloride
7. filtrationwas filtered off
8. customEvaporation at 60° C./25 mbar
9. waitleft a viscous liquid residue (586 g) which
10. additionwas diluted with water (350 ml)
11. customSalts and excess amines were removed by treatment with ion exchangers Amberlite200C (143 ml), IRA67 (148 ml)
12. filtrationIRA900 (56 ml) followed by filtration
13. washThe ion exchangers were washed with water (2×400 ml)
14. additionSome seeding crystals were added to the combined aqueous phase
15. stirringthe solution was stirred slowly at 22° C. for 9 days
16. customThe precipitate was isolated by filtration
17. stirringstirred for 1 day
18. filtrationThe suspension was filtered
19. customthe filter cake was dried in air (216 g, 57% yield, 88.6% HPLC purity)
20. customThe crude product was purified by preparative HPLC (column
21. waithold 10 min
22. customfreeze dried