反应详情

Agilent 1100 with DAD (G1315A), quat. pump (G1311A), autosampler CTC HTS PAL, degasser (G1322A) and column thermostat (G1316A); column: Zorbax Extend-C18 3.5μ; temperature: 40° C.; eluent A: water+5 ml of perchloric acid/liter, eluent B: acetonitrile; flow rate: 0.7 ml/min; gradient: 0-0.5 min 98% A, 2% B; ramp 0.5-4.5 min 10% A, 90% B; 4.5-6 min 10% A, 90% B; ramp 6.5-6.7 min 98% A, 2% B; 6.7-7.5 min 98% A, 2% B.

1. custom40° C.
2. custom0-0.5 min 98% A, 2% B
3. customramp 0.5-4.5 min 10% A, 90% B
4. custom4.5-6 min 10% A, 90% B
5. customramp 6.5-6.7 min 98% A, 2% B
6. custom6.7-7.5 min 98% A, 2% B