HuagongsheHuagongshe Chemistry Print Report · For research reference only
Phosphate → D-(-)-Fructose
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Phosphate | HCID 1061 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Product | D-(-)-Fructose | HCID 5984 | — |
Reaction Steps
To a glucose isomerase solution are added 0.1 M D-glucose, 0.05 M phosphate buffer solution and 0.005 M MgSO4.7H2O and, while maintaining pH at 7.0, the reaction is carried out at 70° C. for one hour. The amount of fructose formed is measured. One unit of activity is expressed as the amount of enzymes necessary for forming 1 mg of fructose under the above conditions.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US04170696 |
| Source dataset | uspto-grants-1979_10 |
| ORD ID | ord-72ad04e6f96c4aaa87711fc8f6e8bdc3 |
| Source note | uspto-grants-1979_10 |