Phosphate → D-(-)-Fructose

HRID 426641Notes

Reaction equation

HRID 426641 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].P([O-])([O-])([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

3

Reactant 2

HCID 1061PhosphateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×

Product 1

HCID 5984D-(-)-Fructose

Procedure

To a glucose isomerase solution are added 0.1 M D-glucose, 0.05 M phosphate buffer solution and 0.005 M MgSO4.7H2O and, while maintaining pH at 7.0, the reaction is carried out at 70° C. for one hour. The amount of fructose formed is measured. One unit of activity is expressed as the amount of enzymes necessary for forming 1 mg of fructose under the above conditions.

Workup

  1. temperaturewhile maintaining pH at 7.0

Sources & evidence

ORD record
ord-72ad04e6f96c4aaa87711fc8f6e8bdc3
Source dataset
uspto-grants-1979_10
Patent number
US04170696

Reaction notes

Loading…