HRID43232

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：C[C@H](N)C(=O)O.NCCCC[C@H](N)C(=O)O.N[C@@H](CCC(=O)O)C(=O)O.N[C@@H](Cc1ccc(O)cc1)C(=O)O

HCID65370

Glatiramer

C23H41N5O11

PRODUCTS

生成物

结构：CC(=O)O.C[C@H](N)C(=O)O.NCCCC[C@H](N)C(=O)O.N[C@@H](CCC(=O)O)C(=O)O.N[C@@H](Cc1ccc(O)cc1)C(=O)O

HCID3081884

Glatiramer acetate

C25H45N5O13

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID517165

Ammonium acetate

C2H7NO2

2 次

PROCEDURE

实验过程

The glatiramer acetate solution prepared in Example 31 is concentrated in vacuo to dryness and subjected to chromatographic separation. A column for gel filtration, FRACTOGEL TSK HW55 (600×26 mm) is prepared in a Superformance 26 Merck cartridge according to the manufacturer's instructions. The column is equilibrated with 0.2 M ammonium acetate buffer pH 5.0, 30 mL of glatiramer free base solution samples (20 mg/mL, in 0.2 M ammonium acetate pH 5.0) are loaded on the column and fractions are collected. A fraction having an average molecular weight of 7-10 kDa is isolated.

WORKUP

后处理

concentrationThe glatiramer acetate solution prepared in Example 31 is concentrated in vacuo to dryness
customsubjected to chromatographic separation
filtrationA column for gel filtration, FRACTOGEL TSK
custom(600×26 mm) is prepared in a Superformance 26 Merck cartridge
customare collected
customis isolated

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-cd7b4602388849a2a28b067a224c6e61
来源数据集: uspto-grants-2013_09
DOI: 10.6084/m9.figshare.5104873.v1
专利: US08536305B2
来源说明: uspto-grants-2013_09

反应 SMILES

[CH3:1][C@H:2]([NH2:6])[C:3]([OH:5])=[O:4].[CH:7]1[C:12]([CH2:13][C@H:14]([NH2:18])[C:15]([OH:17])=[O:16])=[CH:11][CH:10]=[C:9]([OH:19])[CH:8]=1.[CH2:20]([CH2:24][C@H:25]([NH2:29])[C:26]([OH:28])=[O:27])[CH2:21][CH2:22][NH2:23].[CH2:30]([C@H:35]([NH2:39])[C:36]([OH:38])=[O:37])[CH2:31][C:32]([OH:34])=[O:33]>C([O-])(=O)C.[NH4+]>[CH3:1][C@H:2]([NH2:6])[C:3]([OH:5])=[O:4].[CH3:14][C:15]([OH:17])=[O:16].[CH:7]1[C:12]([CH2:13][C@H:14]([NH2:18])[C:15]([OH:17])=[O:16])=[CH:11][CH:10]=[C:9]([OH:19])[CH:8]=1.[CH2:20]([CH2:24][C@H:25]([NH2:29])[C:26]([OH:28])=[O:27])[CH2:21][CH2:22][NH2:23].[CH2:30]([C@H:35]([NH2:39])[C:36]([OH:38])=[O:37])[CH2:31][C:32]([OH:34])=[O:33]