Phosphate → D-(-)-Fructose

Participants

Reaction Conditions

Reaction Steps

A mixture of aqueous solutions of 0.2 ml of 1 M D-glucose 0.2 ml of 0.05 M MgSO4.7H2O and 0.2 ml of 0.5 M phosphate buffer (pH = 7.2) is added to 0.2 ml of glucose isomerase extract and the solution is diluted with water to make 2 ml. The resulting diluted solution is maintained at a temperature of 70° C for 60 minutes to effect isomerization and the reaction is stopped by addition of 2 ml of a 0.5 M aqueous perchloric acid solution. Then the amount of fructose produced is determined by cystein-carbazole method. Then the activity titer expressed by the unit of U is given by the following equation: ##EQU2##

Provenance & Evidence