Phosphate → D-(-)-Fructose
Reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].P([O-])([O-])([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]Participants
3
Conditions
- Temperature
- 70 °C
Procedure
A mixture of aqueous solutions of 0.2 ml of 1 M D-glucose 0.2 ml of 0.05 M MgSO4.7H2O and 0.2 ml of 0.5 M phosphate buffer (pH = 7.2) is added to 0.2 ml of glucose isomerase extract and the solution is diluted with water to make 2 ml. The resulting diluted solution is maintained at a temperature of 70° C for 60 minutes to effect isomerization and the reaction is stopped by addition of 2 ml of a 0.5 M aqueous perchloric acid solution. Then the amount of fructose produced is determined by cystein-carbazole method. Then the activity titer expressed by the unit of U is given by the following equation: ##EQU2##
Workup
- extractionextract
- additionthe solution is diluted with water
- additionthe reaction is stopped by addition of 2 ml of a 0.5 M aqueous perchloric acid solution
Sources & evidence
- ORD record
- ord-0bbc3089a3c74d049eb192ae8b2f52bc
- Source dataset
- uspto-grants-1977_02
- Patent number
- US04008124
Reaction notes
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