Sucrose → Dopamine

Participants

Reaction Steps

The pooled supernatant was underlayed with 16 mL of a 36 % sucrose solution prepared in 10 mM Tris (pH 9.0). The supernatant was then ultracentrifuged at 18,000 rpm in a Beckman SW28 rotor for 80 min at 4° C. to pellet recombinant virus. After ultracentrifugation, the supernatant was removed from the visible virus pellet by aspiration, and the pellet resuspended in 1.0-1.5 mL 10 mm Tris (pH 9.0). This suspension was dounced about 7 times on ice to provide a suspension having a milky, even consistency. The recombinant virus suspension was then transferred in 20-40μL aliquots to 2 mL plastic screw-capped FALCON tubes, titred and stored at -70° C. Typical virus stocks prepared in this way were found to have viral titres of about 1010 pfu/mL.

Provenance & Evidence