Sucrose → Dopamine

HRID 439698Notes

Reaction equation

HRID 439698 reaction equation
C(O)[C@H]1O[C@H](O[C@:9]2([CH2:18]O)[O:13][C@H:12]([CH2:14][OH:15])[C@@H:11](O)[C@@H:10]2O)[C@H](O)[C@@H](O)[C@@H]1O.[CH2:24](O)[C:25]([NH2:30])(CO)CO>>[NH2:30][CH2:25][CH2:24][C:10]1[CH:9]=[CH:18][C:14]([OH:15])=[C:12]([OH:13])[CH:11]=1

Participants

3

Reactant 2

HCID 5988SucroseHCID 6503Tris(hydroxymethyl)aminomethane

Product 1

HCID 681Dopamine

Procedure

The pooled supernatant was underlayed with 16 mL of a 36 % sucrose solution prepared in 10 mM Tris (pH 9.0). The supernatant was then ultracentrifuged at 18,000 rpm in a Beckman SW28 rotor for 80 min at 4° C. to pellet recombinant virus. After ultracentrifugation, the supernatant was removed from the visible virus pellet by aspiration, and the pellet resuspended in 1.0-1.5 mL 10 mm Tris (pH 9.0). This suspension was dounced about 7 times on ice to provide a suspension having a milky, even consistency. The recombinant virus suspension was then transferred in 20-40μL aliquots to 2 mL plastic screw-capped FALCON tubes, titred and stored at -70° C. Typical virus stocks prepared in this way were found to have viral titres of about 1010 pfu/mL.

Workup

  1. customAfter ultracentrifugation, the supernatant was removed from the visible virus pellet by aspiration
  2. customto provide a suspension
  3. customplastic screw-capped FALCON tubes
  4. customstored at -70° C
  5. customTypical virus stocks prepared in this way

Sources & evidence

ORD record
ord-935e539324504ea592ec88e6456fdb3a
Source dataset
uspto-grants-1996_05
Patent number
US05516683

Reaction notes

Loading…