反应详情

D-pantothenic acid was quantified by high performance liquid chromatography [column: Shimadzu SCR101H (7.9 mm dia.×30 cm); mobile phase: 0.008N sulfuric acid; flow rate: 0.8 ml/min; detector: differential refractometer] and/or microbial bioassay [indicator strain: Lactobacillus plantatum IFO 3070; medium: commercially available pantothenic acid assay medium (produced by DIFCO)]. Quantitative determination and optical purity determination of pantoic acid were conducted by high performance liquid chromatography [column: SUMICHIRAL OA-1200; mobile phase: n-hexane/1,2-dichloroethane/ethanol=90/8/2; flow rate: 1.0 ml/min; detector: UV] of the ethyl acetate extract after cell removal from the culture broth by centrifugation, addition of 6N hydrochloric acid to the supernatant and subsequent heating in a water bath at 80° C. for 15 minutes (by this operation, pantoic acid in an equilibrium is converted to pantolactone).