Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Sulfuric Acid | HCID 1118 | — |
| Reactant | L-Pantothenic acid | HCID 5748353 | — |
| Product | Pantothenic Acid | HCID 6613 | — |
Reaction Steps
D-pantothenic acid was quantified by high performance liquid chromatography [column: Shimadzu SCR101H (7.9 mm dia.×30 cm); mobile phase: 0.008N sulfuric acid; flow rate: 0.8 ml/min; detector: differential refractometer] and/or microbial bioassay [indicator strain: Lactobacillus plantatum IFO 3070; medium: commercially available pantothenic acid assay medium (produced by DIFCO)]. Quantitative determination and optical purity determination of pantoic acid were conducted by high performance liquid chromatography [column: SUMICHIRAL OA-1200; mobile phase: n-hexane/1,2-dichloroethane/ethanol=90/8/2; flow rate: 1.0 ml/min; detector: UV] of the ethyl acetate extract after cell removal from the culture broth by centrifugation, addition of 6N hydrochloric acid to the supernatant and subsequent heating in a water bath at 80° C. for 15 minutes (by this operation, pantoic acid in an equilibrium is converted to pantolactone).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US05518906 |
|---|
| Source dataset | uspto-grants-1996_05 |
|---|
| ORD ID | ord-d5f92c09c85c4ea5ad2bd38b354d8e82 |
|---|
| Source note | uspto-grants-1996_05 |
|---|
