HuagongsheHuagongshe Chemistry Print Report · For research reference only
Iodine → Beta-D-Glucose 6-phosphate
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Iodine | HCID 807 | — |
| Reactant | Nadh | HCID 439153 | — |
| Reactant | Sodium Bicarbonate | HCID 516892 | — |
| Reactant | Barium--6-O-phosphonohexose (1/1) | HCID 16219436 | — |
| Solvent | Dimethylformamide | HCID 6228 | — |
| Product | Beta-D-Glucose 6-phosphate | HCID 439427 | — |
Reaction Steps
Following the procedure described above, 10mg of the T4 methyl ester diglycolate in 0.52ml dry DMF was reacted with 1.3mg NHS and 2.3mg ECDI. To 8.7mg G-6-PDH (0.08μ mole) in 1.65ml 1mg/ml sodium bicarbonate was added 5mg G-6-P and 12.5mg NADH, followed by 0.335ml DMF, followed by 9.5μmole ester prepared above in 0.215ml DMF. In 4 hours, a 47% loss in activity was observed. The hapten number was found to be 12 by UV and 13.2 by iodine analysis.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US04040907 |
| Source dataset | uspto-grants-1977_08 |
| ORD ID | ord-c08a2a382b3b4e68af4b574d4ed770bf |
| Source note | uspto-grants-1977_08 |